Limitation of (1→3)-β-D-glucan monitoring in major elective surgery involving cardiopulmonary bypass

cated for detection of invasive candidiasis. Our data indicate that the rate o f false positive results is potentially high in some patient collectives of risk; for example, after cardiac artery bypass grafting (CABG). Th e recently published guidelines from the Surviving Sepsis Campaign recommend the use of (13)-β-dglucan as a surrogate for invasive candidiasis [1]. Fung al infections are increasing, are underestimated by conventional culture, and are associated with high mortality [2,3]. Testing for fungal wall constituents can detect candidiasis early, but its additional value in critically ill patients remains unclear [4]. We investigated (13)-β-dglucan levels in patients with sepsis and compare the values with those of postoperative patients after CABG, refl ecting a well-defi ned ICU cohort. After institutional approval by Local Ethics Committee Jena, 21 patients (aged ≥18 years) with sepsis, severe sepsis or septic shock according to American College of Chest Physicians/Society of Critical Care Medicine criteria, 23 patients after onpump CABG as well as 21 healthy controls were enrolled. All patients or legal surrogates gave informed consent. Patients’ charac teristics are presented in Table 1. Blood sampling was performed in patients on the day of diagnosis (sepsis) or on the fi rst postoperative day (CABG). Th e measurement of (13)-β-d-glucan was per formed by WAKO Inc. (Osaka, Japan). We found increased (13)-β-d-glucan levels in patients with sepsis compared with healthy controls (Figure 1), but patients after CABG exhibited the highest median (13)-β-d-glucan values; none developed signs of invasive candidiasis and the median ICU length of stay was 1 day. Eleven of 18 (61%) patients with sepsis reached (13)-β-d-glucan levels above the upper limit of normal (11 pg/ml). An incidence rate of 61% for invasive candi diasis appears very high [2], suggesting that cutoff values in the ICU setting where translocation might occur warrant reappraisal. Similarly, eight out of 18 (44%) patients after CABG presented elevated levels. Since the highest levels of (13)-β-d-glucan were found in CABG patients after onpump surgery, we assume – similarly to patients undergoing haemodialysis [5] – that (13)-β-d-glucan might have derived from membranes during onpump surgery rather than from infection or translocation. Since elevated (13)-β-d-glucan levels in © 2010 BioMed Central Ltd Limitation of (13)-β-D-glucan monitoring in major elective surgery involving cardiopulmonary bypass